Journal: NMR in biomedicine
Article Title: Magnetic resonance imaging of hypoxia in acute stroke compared with fluorine-18 fluoromisonidazole-positron emission tomography: A cross-validation study?
doi: 10.1002/nbm.4858
Figure Lengend Snippet: FIGURE 2 (A) Representative images obtained in one rat at 3 h following the MCAo. The ADC map depicts the core of ischemia (arrow). The time-to-peak map reveals a larger zone of hypoperfusion (arrow). The penumbra was delineated as the mismatch between perfusion and diffusion abnormalities (hatched purple). The StO2-MRI map shows the area with reduced oxygen saturation (arrow). [18F]-FMISO-PET delineates the hypoxic area with increased retention of [18F]-FMISO (arrow). The T2-W image, acquired 24 h after the MCAo, shows the consolidated lesion (arrow). (B) Evolution of ADC-defined lesion volumes. The lesion demonstrable at 15 min expanded at 3 h, and even more so at 24 h following the MCAo. **p < 0.01; ***p < 0.001; ANOVA with repeated measures followed by Tukey's post hoc test. The boxes define the interquartile range; the solid horizontal line the median, and the whiskers represent the minimal and maximal values. (C) Volumes of brain compartments at 3 h following the MCAo. Time-to-peak (TTP): tissue volume with decreased perfusion; Penumbra: tissue with PWI/DWI mismatch; StO2-MRI: tissue that displays a decrease in oxygen saturation; [18F]-FMISO-PET: tissue volume that shows an increased retention of [18F]-FMISO. The boxes define the interquartile range; the solid horizontal line the median and the whiskers represent the minimal and maximal values. ADC, apparent diffusion coefficient; ANOVA, analysis of variance; DWI, diffusion-weighted imaging; MCAo, middle cerebral artery occlusion; PET, positron emission tomography; PWI, perfusion-weighted imaging; StO2-MRI, magnetic resonance-based imaging of brain oxygen saturation; T2-W, T2-weighted; [18F]-FMISO, 18-fluorine-fluoromisonidazole
Article Snippet: The following parameters were employed: resolution = 0.3 x 0.3 x 1.5 mm, FOV = 31.5 x 15 x 15 mm; TR/TE = 3000/46.4 ms; b-values: 0 and 1000 s/mm2; 30 directions; number of experiments (NEX) = 3; and acquisi- tion time: 5 min 15 s. Apparent diffusion coefficient (ADC) maps were then generated with PV 5.1 software (Bruker).
Techniques: Diffusion-based Assay, Imaging, Positron Emission Tomography, Magnetic Resonance Imaging
![FIGURE 2 (A) Representative images obtained in one rat at 3 h following the MCAo. The <t>ADC</t> map depicts the core of ischemia (arrow). The time-to-peak map reveals a larger zone of hypoperfusion (arrow). The penumbra was delineated as the mismatch between perfusion and diffusion abnormalities (hatched purple). The StO2-MRI map shows the area with reduced oxygen saturation (arrow). [18F]-FMISO-PET delineates the hypoxic area with increased retention of [18F]-FMISO (arrow). The T2-W image, acquired 24 h after the MCAo, shows the consolidated lesion (arrow). (B) Evolution of ADC-defined lesion volumes. The lesion demonstrable at 15 min expanded at 3 h, and even more so at 24 h following the MCAo. **p < 0.01; ***p < 0.001; ANOVA with repeated measures followed by Tukey's post hoc test. The boxes define the interquartile range; the solid horizontal line the median, and the whiskers represent the minimal and maximal values. (C) Volumes of brain compartments at 3 h following the MCAo. Time-to-peak (TTP): tissue volume with decreased perfusion; Penumbra: tissue with PWI/DWI mismatch; StO2-MRI: tissue that displays a decrease in oxygen saturation; [18F]-FMISO-PET: tissue volume that shows an increased retention of [18F]-FMISO. The boxes define the interquartile range; the solid horizontal line the median and the whiskers represent the minimal and maximal values. ADC, apparent diffusion <t>coefficient;</t> ANOVA, analysis of variance; DWI, diffusion-weighted imaging; MCAo, middle cerebral artery occlusion; PET, positron emission tomography; PWI, perfusion-weighted imaging; StO2-MRI, magnetic resonance-based imaging of brain oxygen saturation; T2-W, T2-weighted; [18F]-FMISO, 18-fluorine-fluoromisonidazole](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_5719/pm36285719/pm36285719__page5_image1.jpg)
![FIGURE 5 Representative images of StO2-MRI, [18F]-FMISO-PET, and the superimposition of both abnormalities on the ADC image for each animal. StO2-MRI abnormality is delinated in yellow and [18F]-FMISO-PET abnormality in green. ADC, apparent diffusion coefficient; [18F]-FMISO, 18-fluorine-fluoromisonidazole; PET, positron emission tomography; StO2-MRI, magnetic resonance-based imaging of brain oxygen saturation; SUV, standardized uptake value](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_5719/pm36285719/pm36285719__page8_image1.jpg)